Detection of Chymotrypsin by Optical and Acoustic Methods

Chymotrypsin is an important proteolytic enzyme in the human digestive system that cleaves milk proteins through hydrolysis reaction, making it interesting subject to study activity of proteases. In this work, we compared detection chymotrypsin by spectrophotometric dynamic light scattering (DLS) and quartz crystal microbalance (QCM) methods determined limit (LOD), 0.15 ± 0.01 nM for spectrophotometric, 0.67 0.05 DLS 1.40 0.30 QCM methods, respectively. The sensors are relatively cheap able detect 3035 min. While optical simple implement, method more robust sample preparation, allows non-transparent samples. We give overview on instruments other